منابع مشابه
Carbonic anhydrase in human platelets.
The carbonic anhydrase activity of human platelets was investigated by measuring the kinetics of CO2 hydration in supernatants of platelet lysates by using a pH stopped-flow apparatus. An average carbonic anhydrase concentration of 2.1 microM was determined for pellets of human platelets. Analysis of the kinetic properties of this carbonic anhydrase yielded a Km value of 1.0 mM, a catalytic-cen...
متن کاملNucleotide sequence of a cDNA encoding rice chloroplastic carbonic anhydrase.
CA (EC 4.2.1.1) catalyzes the reversible hydration of CO, according to the reaction CO, + H,O HCO, + H+ and represents 1 to 2% of total leaf soluble protein (Okabe et al., 1984). Considerable differences have been reported to exist between the molecular mass of the holoenzyme in monocotyledonous and dicotyledonous species (4245 kD in monocotyledonous plants and between 140 and 250 kD in dicotyl...
متن کاملSequence of a cDNA encoding carbonic anhydrase from barley.
CA catalyzes the reversible hydration of CO, to generate bicarbonate and a proton. Although its physiological function has not been clearly defined, it had been speculated that CA serves to concentrate CO, at the active site of Rubisco (Graham et al., 1984). However, recent work with transgenic plants expressing CA in the antisense orientation has shown that the enzyme does not play an essentia...
متن کاملCloning and Expression of Brassica napus β-Carbonic Anhydrase cDNA
Carbonic anhydrase (CA, carbonate dehydratase, carbonate hydrolyase; EC 4.2.1.1) is a zinccontaining enzyme that catalyzes the reversible hydration of CO2 (CO2 + H2O HCO3 – + H) (Khalifah, 1971). CA is one of the enzymes whose catalytic velocities are confi rmed to be the fastest. It is widely distributed in animals, plants, archaebacteria and eubacteria (Hewett-Emmett and Tashian, 1996). The k...
متن کاملCharacterisation of cDNA clones for rat muscle carbonic anhydrase III.
cDNA clones for rat muscle carbonic anhydrase III have been isolated from a lambda gt-11 library and sequenced. Comparison with human CAIII cDNA showed about 90% homology to rat. The rat clones were used to estimate mRNA from liver and muscle on Northern blots and showed that the sexual dimorphism of CAIII in rat liver relates to a difference in mRNA levels.
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1987
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/15.5.2386